Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase
نویسندگان
چکیده
منابع مشابه
Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.
Encephalomyocarditis (EMC) virus RNA-dependent RNA polymerase was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST), which allowed easy purification of the fusion protein by affinity chromatography on immobilized glutathione. Inclusion of a thrombin cleavage site between the GST carrier and the viral enzyme facilitated the release of purified mature EMC viru...
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Small amounts of encephalomyocarditis virus RNA direct a 50-fold increase in amino acid incorporation, in appropriately supplemented ascites tumor cell extracts, under conditions that give rise to authentic viral polypeptides. Incorporation in these crude extracts has a novel characteristic, namely, that it is almost entirely dependent upon the addition of exogenous tRNA. Further, this incorpor...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1991
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.65.6.2993-3000.1991